Penicillium citrinum UFV1 β-glucosidases: purification, characterization, and application for biomass saccharification
نویسندگان
چکیده
منابع مشابه
Purification of endoglucanase produced by Penicillium citrinum isolated from Amazon
Background The cellulolytic enzyme complex has many important biotechnological applications such as beverage, textile, food, paper and cellulose industries, as well as in degradation of lignocellulosic for ethanol biofuel production [1]. According to the enzymatic activity, cellulolytic complex is subdivided in three classes: endoglucanases, exoglucanases and b-D-glycosidases. Endoglucanases (e...
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An extracellular thermostable xylanase (Xyl-IIb) produced by Penicillium citrinum isolate HZN13 was purified to homogeneity using DEAE-Sepharose, Sephadex G-100 and Bio-Gel P-60 chromatography with specific activity of 6272.7 U/mg and 19.6-fold purification. The purification revealed the occurrence of multiple forms of xylanases (Xyl-I, Xyl-IIa, Xyl-IIb and Xyl-III). The molecular mass of highl...
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BACKGROUND Enzyme end-product inhibition is a major challenge in the hydrolysis of lignocellulose at a high dry matter consistency. β-glucosidases (BGs) hydrolyze cellobiose into two molecules of glucose, thereby relieving the product inhibition of cellobiohydrolases (CBHs). However, BG inhibition by glucose will eventually lead to the accumulation of cellobiose and the inhibition of CBHs. Ther...
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Very few studies have been dedicated to R-hydroxyacids (R-HA) production using extracellular polyhydroxyalkanoate depolymerases (ePhaZs). Penicillium expansum produced maximum extracellular polyhydroxybutyrate depolymerase (~6 U/mL) by 72 h when grown in mineral salt medium containing 0.2 % w/v PHB, pH 5.0, at 30 °C and 200 rpm shaking conditions. Partial purification of the extracellular poly(...
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سوسک برگخوار بید Plagiodera versicolora یکی از آفات مهم درختان جنگلی در دنیا از جمله ایران میباشد. در پژوهش حاضر، فعالیت آلفا و بتا- گلوکوزیداز در معده میانی حشره کاملP. versicolora بررسی شد. نتایج نشان داد که فعالیت آلفا- گلوکوزیداز بیشتر از بتا-گلوکوزیداز بود. pH بهینه برای فعالیت آلفا و بتا- گلوکوزیداز بهترتیب 6 و 9 بهدست آمد. همچنین دمای بهینه فعالیت این دو آنزیم بهترتیب 30 و 25 درجه...
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ژورنال
عنوان ژورنال: Biotechnology for Biofuels
سال: 2018
ISSN: 1754-6834
DOI: 10.1186/s13068-018-1226-5